|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Human EFNB1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10894-ACG|
|Human EFNB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10894-ACR|
|Human EFNB1 ORF mammalian expression plasmid, C-Flag tag||HG10894-CF|
|Human EFNB1 ORF mammalian expression plasmid, C-His tag||HG10894-CH|
|Human EFNB1 ORF mammalian expression plasmid, C-Myc tag||HG10894-CM|
|Human EFNB1 ORF mammalian expression plasmid, C-HA tag||HG10894-CY|
|Human EFNB1 Gene cDNA clone plasmid||HG10894-M|
|Human EFNB1 ORF mammalian expression plasmid, N-Flag tag||HG10894-NF|
|Human EFNB1 ORF mammalian expression plasmid, N-His tag||HG10894-NH|
|Human EFNB1 ORF mammalian expression plasmid, N-Myc tag||HG10894-NM|
|Human EFNB1 ORF mammalian expression plasmid, N-HA tag||HG10894-NY|
|Human EFNB1 natural ORF mammalian expression plasmid||HG10894-UT|
|Learn more about expression Vectors|
Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.