|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Human ECE-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10887-ACG|
|Human ECE-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10887-ACR|
|Human ECE-1 ORF mammalian expression plasmid, C-Flag tag||HG10887-CF|
|Human ECE-1 ORF mammalian expression plasmid, C-His tag||HG10887-CH|
|Human ECE-1 ORF mammalian expression plasmid, C-Myc tag||HG10887-CM|
|Human ECE-1 ORF mammalian expression plasmid, C-HA tag||HG10887-CY|
|Human ECE-1 Gene cDNA clone plasmid||HG10887-M|
|Human ECE-1 ORF mammalian expression plasmid, N-Flag tag||HG10887-NF|
|Human ECE-1 ORF mammalian expression plasmid, N-His tag||HG10887-NH|
|Human ECE-1 ORF mammalian expression plasmid, N-Myc tag||HG10887-NM|
|Human ECE-1 ORF mammalian expression plasmid, N-HA tag||HG10887-NY|
|Human ECE-1 natural ORF mammalian expression plasmid||HG10887-UT|
|Learn more about expression Vectors|
Endothelin-converting enzyme 1, also known as ECE-1, is a single-pass type I I membrane protein which belongs to the peptidase M13 family. ECE-1 converts big endothelin-1 to endothelin-1. ECE-1 is a membrane metalloprotease that generates endothelin from its direct precursor big endothelin. Four isoforms of ECE-1 are produced from a single gene through the use of alternate promoters. These isoforms share the same extracellular catalytic domain and contain unique cytosolic tails, which results in their specific subcellular targeting.All isoforms of ECE-1 are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C of ECE-1 are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C of ECE-1 in liver, testis and small intestine; isoform B, C and D of ECE-1 in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Defects in ECE1 are a cause of Hirschsprung disease, cardiac defects and autonomic dysfunction. It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction.