|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human B3GNT6 ORF mammalian expression plasmid, C-GFPSpark tag||HG13951-ACG|
|Human B3GNT6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13951-ACR|
|Human B3GNT6 ORF mammalian expression plasmid, C-Flag tag||HG13951-CF|
|Human B3GNT6 ORF mammalian expression plasmid, C-His tag||HG13951-CH|
|Human B3GNT6 ORF mammalian expression plasmid, C-Myc tag||HG13951-CM|
|Human B3GNT6 ORF mammalian expression plasmid, C-HA tag||HG13951-CY|
|Human B3GNT6 Gene cDNA clone plasmid||HG13951-G|
|Human B3GNT6 ORF mammalian expression plasmid, N-Flag tag||HG13951-NF|
|Human B3GNT6 ORF mammalian expression plasmid, N-His tag||HG13951-NH|
|Human B3GNT6 ORF mammalian expression plasmid, N-Myc tag||HG13951-NM|
|Human B3GNT6 ORF mammalian expression plasmid, N-HA tag||HG13951-NY|
|Human B3GNT6 natural ORF mammalian expression plasmid||HG13951-UT|
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B3GNT6 belongs to the glycosyltransferase 31 family. B3GNT6 play s an important role in the synthesis of mucin-type O-glycans in digestive organs. It catalyzes the transfer of GlcNAc from UDP-GlcNAc to GalNAcalpha1-Ser/Thr (Tn antigen) to form the core 3 structure (GlcNAcbeta1-3GalNAcalpha1-Ser/Thr). Core 3 structure exists in O-glycan which is an important precursor in the biosynthesis of mucin-type glycoproteins. Loss of core 3 could lead to the production of secreted mucins, then bacteria would be inefficiently cleared from the system, and chronic inflammation would be developed, which eventually would result in development of cancer. B3GNT6 gene is a tumor suppressor gene.