|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PLBD2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13947-ACG|
|Human PLBD2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13947-ACR|
|Human PLBD2 ORF mammalian expression plasmid, C-Flag tag||HG13947-CF|
|Human PLBD2 ORF mammalian expression plasmid, C-His tag||HG13947-CH|
|Human PLBD2 ORF mammalian expression plasmid, C-Myc tag||HG13947-CM|
|Human PLBD2 ORF mammalian expression plasmid, C-HA tag||HG13947-CY|
|Human PLBD2 Gene cDNA clone plasmid||HG13947-G|
|Human PLBD2 ORF mammalian expression plasmid, His tag||HG13947-G-H|
|Human PLBD2 ORF mammalian expression plasmid, N-Flag tag||HG13947-NF|
|Human PLBD2 ORF mammalian expression plasmid, N-His tag||HG13947-NH|
|Human PLBD2 ORF mammalian expression plasmid, N-Myc tag||HG13947-NM|
|Human PLBD2 ORF mammalian expression plasmid, N-HA tag||HG13947-NY|
|Human PLBD2 natural ORF mammalian expression plasmid||HG13947-UT|
|Learn more about expression Vectors|
PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.