|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human KLK6 ORF mammalian expression plasmid, C-GFPSpark tag||HG12142-ACG|
|Human KLK6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12142-ACR|
|Human KLK6 ORF mammalian expression plasmid, C-Flag tag||HG12142-CF|
|Human KLK6 ORF mammalian expression plasmid, C-His tag||HG12142-CH|
|Human KLK6 ORF mammalian expression plasmid, C-Myc tag||HG12142-CM|
|Human KLK6 ORF mammalian expression plasmid, C-HA tag||HG12142-CY|
|Human KLK6 Gene cDNA clone plasmid||HG12142-G|
|Human KLK6 ORF mammalian expression plasmid, N-Flag tag||HG12142-NF|
|Human KLK6 ORF mammalian expression plasmid, N-His tag||HG12142-NH|
|Human KLK6 ORF mammalian expression plasmid, N-Myc tag||HG12142-NM|
|Human KLK6 ORF mammalian expression plasmid, N-HA tag||HG12142-NY|
|Human KLK6 natural ORF mammalian expression plasmid||HG12142-UT|
|Learn more about expression Vectors|
KLK6 (kallikrein-related peptidase 6), also known as Klk7, belongs to the peptidase S1 family, Kallikrein subfamily. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. KLK6 is a serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Klk7 shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. KLK6 degrades alpha-synuclein and prevents its polymerization, indicating that KLK6 may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. Klk7 may be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.