|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human AMIGO2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13735-ACG|
|Human AMIGO2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13735-ACR|
|Human AMIGO2 ORF mammalian expression plasmid, C-Flag tag||HG13735-CF|
|Human AMIGO2 ORF mammalian expression plasmid, C-His tag||HG13735-CH|
|Human AMIGO2 ORF mammalian expression plasmid, C-Myc tag||HG13735-CM|
|Human AMIGO2 ORF mammalian expression plasmid, C-HA tag||HG13735-CY|
|Human AMIGO2 Gene cDNA clone plasmid||HG13735-G|
|Human AMIGO2 ORF mammalian expression plasmid, N-Flag tag||HG13735-NF|
|Human AMIGO2 ORF mammalian expression plasmid, N-His tag||HG13735-NH|
|Human AMIGO2 ORF mammalian expression plasmid, N-Myc tag||HG13735-NM|
|Human AMIGO2 ORF mammalian expression plasmid, N-HA tag||HG13735-NY|
|Human AMIGO2 natural ORF mammalian expression plasmid||HG13735-UT|
|Learn more about expression Vectors|
AMIGO2 contains Ig-like C2-type (immunoglobulin-like) domain, 6 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. It belongs to the immunoglobulin superfamily, AMIGO family. AMIGO2 may mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. It is required for depolarization-dependent survival of cultured cerebellar granule neurons. AMIGO2 may also contribute to signal transduction through its intracellular domain. It may play a role in the tumorigenesis of a subset of gastric adenocarcinomas. AMIGO2 is highly expressed in breast, ovary, cervix, and uterus.