|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human HMGN3 ORF mammalian expression plasmid, C-GFPSpark tag||HG13914-ACG|
|Human HMGN3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13914-ACR|
|Human HMGN3 ORF mammalian expression plasmid, N-GFPSpark tag||HG13914-ANG|
|Human HMGN3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13914-ANR|
|Human HMGN3 ORF mammalian expression plasmid, C-Flag tag||HG13914-CF|
|Human HMGN3 ORF mammalian expression plasmid, C-His tag||HG13914-CH|
|Human HMGN3 ORF mammalian expression plasmid, C-Myc tag||HG13914-CM|
|Human HMGN3 ORF mammalian expression plasmid, C-HA tag||HG13914-CY|
|Human HMGN3 Gene cDNA clone plasmid||HG13914-G|
|Human HMGN3 ORF mammalian expression plasmid, N-Flag tag||HG13914-NF|
|Human HMGN3 ORF mammalian expression plasmid, N-His tag||HG13914-NH|
|Human HMGN3 ORF mammalian expression plasmid, N-Myc tag||HG13914-NM|
|Human HMGN3 ORF mammalian expression plasmid, N-HA tag||HG13914-NY|
|Human HMGN3 natural ORF mammalian expression plasmid||HG13914-UT|
|Learn more about expression Vectors|
HMGN3 belongs to the HMGN family and is expressed in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. Members of the HMGN family bind to nucleosomes without any specificity for the underlying DNA sequence. They affect the global and local structure of chromatin, as well as the levels of histone modifications and thus play a role in epigenetic regulation of gene expression. HMGN3 regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. It also regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. Both insulin and glucagon levels can be affected by HMGN3. HMGN3 may play a role in ocular development and astrocyte function. It also modulates the expression of pancreatic genes involved in insulin secretion.