|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human FH ORF mammalian expression plasmid, C-GFPSpark tag||HG12115-ACG|
|Human FH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12115-ACR|
|Human FH ORF mammalian expression plasmid, N-GFPSpark tag||HG12115-ANG|
|Human FH ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12115-ANR|
|Human FH ORF mammalian expression plasmid, C-Flag tag||HG12115-CF|
|Human FH ORF mammalian expression plasmid, C-His tag||HG12115-CH|
|Human FH ORF mammalian expression plasmid, C-Myc tag||HG12115-CM|
|Human FH ORF mammalian expression plasmid, C-HA tag||HG12115-CY|
|Human FH Gene cDNA clone plasmid||HG12115-M|
|Human FH ORF mammalian expression plasmid, N-Flag tag||HG12115-NF|
|Human FH ORF mammalian expression plasmid, N-His tag||HG12115-NH|
|Human FH ORF mammalian expression plasmid, N-Myc tag||HG12115-NM|
|Human FH ORF mammalian expression plasmid, N-HA tag||HG12115-NY|
|Human FH natural ORF mammalian expression plasmid||HG12115-UT|
|Learn more about expression Vectors|
Fumarate Hydratase (FH) is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. Fumarate Hydratase is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. Individuals with hemizygous germline fumarate hydratase (FH) mutations are predisposed to renal cancer. These tumors predominantly exhibit functional inactivation of the remaining wild-type allele, implicating FH inactivation as a tumor-promoting event.