|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LYZL2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13726-ACG|
|Human LYZL2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13726-ACR|
|Human LYZL2 ORF mammalian expression plasmid, C-Flag tag||HG13726-CF|
|Human LYZL2 ORF mammalian expression plasmid, C-His tag||HG13726-CH|
|Human LYZL2 ORF mammalian expression plasmid, C-Myc tag||HG13726-CM|
|Human LYZL2 ORF mammalian expression plasmid, C-HA tag||HG13726-CY|
|Human LYZL2 Gene cDNA clone plasmid||HG13726-G|
|Human LYZL2 ORF mammalian expression plasmid, N-Flag tag||HG13726-NF|
|Human LYZL2 ORF mammalian expression plasmid, N-His tag||HG13726-NH|
|Human LYZL2 ORF mammalian expression plasmid, N-Myc tag||HG13726-NM|
|Human LYZL2 ORF mammalian expression plasmid, N-HA tag||HG13726-NY|
|Human LYZL2 natural ORF mammalian expression plasmid||HG13726-UT|
|Learn more about expression Vectors|
Lysozyme 2 gene is a member of a family of lysozyme-like genes. Lysozymes, especially C-type lysozymes, are well-recognized bacteriolytic factors widely distributed in the animal kingdom and play a mainly protective role in host defense. Lysozymes damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Lysozyme is part of the innate immune system. Reduced lysozyme levels have been associated with bronchopulmonary dysplasia in newborns. In certain cancers (especially myelomonocytic leukemia) excessive production of lysozyme by cancer cells can lead to toxic levels of lysozyme in the blood. High lysozyme blood levels can lead to kidney failure and low blood potassium, conditions that may improve or resolve with treatment of the primary malignancy.