|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SCN3B ORF mammalian expression plasmid, C-GFPSpark tag||HG13897-ACG|
|Human SCN3B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13897-ACR|
|Human SCN3B ORF mammalian expression plasmid, C-Flag tag||HG13897-CF|
|Human SCN3B ORF mammalian expression plasmid, C-His tag||HG13897-CH|
|Human SCN3B ORF mammalian expression plasmid, C-Myc tag||HG13897-CM|
|Human SCN3B ORF mammalian expression plasmid, C-HA tag||HG13897-CY|
|Human SCN3B Gene cDNA clone plasmid||HG13897-G|
|Human SCN3B ORF mammalian expression plasmid, N-Flag tag||HG13897-NF|
|Human SCN3B ORF mammalian expression plasmid, N-His tag||HG13897-NH|
|Human SCN3B ORF mammalian expression plasmid, N-Myc tag||HG13897-NM|
|Human SCN3B ORF mammalian expression plasmid, N-HA tag||HG13897-NY|
|Human SCN3B natural ORF mammalian expression plasmid||HG13897-UT|
|Learn more about expression Vectors|
SCN3B (sodium channel, voltage-gated, type III, beta ,human IgG1-Fc chimera) belongs to the sodium channel auxiliary subunit SCN3B family. It contains 1 Ig-like C2-type (immunoglobulin-like) domain. Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. SCN3B gene encodes one member of the sodium channel beta subunit gene family, and influences the inactivation kinetics of the sodium channel. Two alternatively spliced variants, encoding the same protein, have been identified. Defects in SCN3B are the cause of Brugada syndrome type 7. A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram. It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset.