|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human GPHA2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13894-ACG|
|Human GPHA2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13894-ACR|
|Human GPHA2 ORF mammalian expression plasmid, C-Flag tag||HG13894-CF|
|Human GPHA2 ORF mammalian expression plasmid, C-His tag||HG13894-CH|
|Human GPHA2 ORF mammalian expression plasmid, C-Myc tag||HG13894-CM|
|Human GPHA2 ORF mammalian expression plasmid, C-HA tag||HG13894-CY|
|Human GPHA2 Gene cDNA clone plasmid||HG13894-G|
|Human GPHA2 ORF mammalian expression plasmid, N-Flag tag||HG13894-NF|
|Human GPHA2 ORF mammalian expression plasmid, N-His tag||HG13894-NH|
|Human GPHA2 ORF mammalian expression plasmid, N-Myc tag||HG13894-NM|
|Human GPHA2 ORF mammalian expression plasmid, N-HA tag||HG13894-NY|
|Human GPHA2 natural ORF mammalian expression plasmid||HG13894-UT|
|Learn more about expression Vectors|
GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activates human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named as thyrostimulin based on its thyroid-stimulating activity. The expression of thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism.