|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human APLP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13716-ACG|
|Human APLP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13716-ACR|
|Human APLP1 ORF mammalian expression plasmid, C-Flag tag||HG13716-CF|
|Human APLP1 ORF mammalian expression plasmid, C-His tag||HG13716-CH|
|Human APLP1 ORF mammalian expression plasmid, C-Myc tag||HG13716-CM|
|Human APLP1 ORF mammalian expression plasmid, C-HA tag||HG13716-CY|
|Human APLP1 Gene cDNA clone plasmid||HG13716-G|
|Human APLP1 ORF mammalian expression plasmid, N-Flag tag||HG13716-NF|
|Human APLP1 ORF mammalian expression plasmid, N-His tag||HG13716-NH|
|Human APLP1 ORF mammalian expression plasmid, N-Myc tag||HG13716-NM|
|Human APLP1 ORF mammalian expression plasmid, N-HA tag||HG13716-NY|
|Human APLP1 natural ORF mammalian expression plasmid||HG13716-UT|
|Learn more about expression Vectors|
APLP1, also known as amyloid-like protein 1, is a member of the highly conserved amyloid precursor protein gene family. APLP1 is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. APLP1, together with APLP2, are important modulators of glucose. APLP1 may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. APLP1 also is a mammalian homologue of amyloid precursor protein (APP). APP is a type I membrane protein that is genetically linked to Alzheimer's disease.