|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human AGA ORF mammalian expression plasmid, C-GFPSpark tag||HG13705-ACG|
|Human AGA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13705-ACR|
|Human AGA ORF mammalian expression plasmid, C-Flag tag||HG13705-CF|
|Human AGA ORF mammalian expression plasmid, C-His tag||HG13705-CH|
|Human AGA ORF mammalian expression plasmid, C-Myc tag||HG13705-CM|
|Human AGA ORF mammalian expression plasmid, C-HA tag||HG13705-CY|
|Human AGA Gene cDNA clone plasmid||HG13705-G|
|Human AGA ORF mammalian expression plasmid, N-Flag tag||HG13705-NF|
|Human AGA ORF mammalian expression plasmid, N-His tag||HG13705-NH|
|Human AGA ORF mammalian expression plasmid, N-Myc tag||HG13705-NM|
|Human AGA ORF mammalian expression plasmid, N-HA tag||HG13705-NY|
|Human AGA natural ORF mammalian expression plasmid||HG13705-UT|
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S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.
Human Protein S100-A8, also known as S100 calcium-binding protein A8, Cystic fibrosis antigen, Migration inhibitory factor-related protein 8, S100A8, and CAGA, is a member of the S-100 family. S100A8 plays a role in various functions of myeloid cells by forming a heterocomplex with S100A9. S100A8 and S100A9 are known to be overexpressed in certain species of carcinomas. S100A8 plays an important role in dedifferentiation of thyroid carcinoma possibly by forming a complex with S100A9. S100A8 and S100A9 may also play a key role in inflammation-associated cancer.