|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SNCA ORF mammalian expression plasmid, C-GFPSpark tag||HG12093-ACG|
|Human SNCA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12093-ACR|
|Human SNCA ORF mammalian expression plasmid, N-GFPSpark tag||HG12093-ANG|
|Human SNCA ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12093-ANR|
|Human SNCA ORF mammalian expression plasmid, C-Flag tag||HG12093-CF|
|Human SNCA ORF mammalian expression plasmid, C-His tag||HG12093-CH|
|Human SNCA ORF mammalian expression plasmid, C-Myc tag||HG12093-CM|
|Human SNCA ORF mammalian expression plasmid, C-HA tag||HG12093-CY|
|Human SNCA Gene cDNA clone plasmid||HG12093-G|
|Human SNCA ORF mammalian expression plasmid, N-Flag tag||HG12093-NF|
|Human SNCA ORF mammalian expression plasmid, N-His tag||HG12093-NH|
|Human SNCA ORF mammalian expression plasmid, N-Myc tag||HG12093-NM|
|Human SNCA ORF mammalian expression plasmid, N-HA tag||HG12093-NY|
|Human SNCA natural ORF mammalian expression plasmid||HG12093-UT|
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Alpha-Synuclein (alpha-Syn), also known as NACP or SNCA, exists as at least two structural isoforms: one is helix-rich, membrane-bound form that both the N- and C-terminal regions of alpha-synuclein are tightly associated with membranes and the other is disordered, cytosolic form. Synuclein is found predominantly in the presynaptic termini, in both free or membrane-bound forms. SNCA is extensively localized in nucleus of neurons. It has been shown that alpha-Synuclein was highly expressed in the mitochondria in olfactory bulb, hippocampus, striatum, and thalamus, where the cytosolic alpha-Synuclein was also rich. Normally the unstructured soluble type of alpha-synuclein can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy bodies and multiple system atrophy. SNCA abnormality and mitochondrial deficiency are two major changes in the brain of patients with Parkinson's disease (PD). In addition, alpha-synuclein is an abundant component of Lewy bodies in sporadic Parkinson's disease and diffuse Lewy body disease.