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Human PRSS1 ORF mammalian expression plasmid, C-His tag

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Human PRSS1 cDNA Clone Product Information
Gene_bank_ref_id:NM_002769.4
RefSeq ORF Size:744bp
cDNA Description:Full length Clone DNA of Homo sapiens protease, serine, 1 (trypsin 1) with C terminal His tag.
Gene Synonym:TRP1, TRY1, TRY4, TRYP1, MGC120175, MGC149362, PRSS1
Species:Human
Vector:pCMV3-C-His
Plasmid:pCMV3-PRSS1-His
Restriction Site:KpnI + XbaI (6kb + 0.79kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 486 T/C not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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Catalog: HG10816-CH
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