|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human MSN ORF mammalian expression plasmid, C-GFPSpark tag||HG13866-ACG|
|Human MSN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13866-ACR|
|Human MSN ORF mammalian expression plasmid, N-GFPSpark tag||HG13866-ANG|
|Human MSN ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13866-ANR|
|Human MSN ORF mammalian expression plasmid, C-Flag tag||HG13866-CF|
|Human MSN ORF mammalian expression plasmid, C-His tag||HG13866-CH|
|Human MSN ORF mammalian expression plasmid, C-Myc tag||HG13866-CM|
|Human MSN ORF mammalian expression plasmid, C-HA tag||HG13866-CY|
|Human MSN Gene cDNA clone plasmid||HG13866-G|
|Human MSN ORF mammalian expression plasmid, N-Flag tag||HG13866-NF|
|Human MSN ORF mammalian expression plasmid, N-His tag||HG13866-NH|
|Human MSN ORF mammalian expression plasmid, N-Myc tag||HG13866-NM|
|Human MSN ORF mammalian expression plasmid, N-HA tag||HG13866-NY|
|Human MSN natural ORF mammalian expression plasmid||HG13866-UT|
|Learn more about expression Vectors|
Moesin is a member of the ERM family which includes ezrin and radixin. ERM proteins, highly related members of the larger protein 4.1 superfamily, can exist in an active or inactive conformation. It seems that ERM proteins function as cross-linkers between plasma membranes and actin-based cytoskeletons. The sole Drosophila ERM protein, moesin, functions to promote cortical actin assembly and apical-basal polarity. As a result, cells lacking moesin lose epithelial characteristics and adopt invasive migratory behaviour. It is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. Moesin contains 1 FERM domain and is expressed in all tissues and cultured cells studied. Moesin has been shown to interact with CD43, Neutrophil cytosolic factor 1, VCAM-1, Neutrophil cytosolic factor 4, ICAM3 and EZR.