|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CAT ORF mammalian expression plasmid, C-GFPSpark tag||HG12084-ACG|
|Human CAT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12084-ACR|
|Human CAT ORF mammalian expression plasmid, N-GFPSpark tag||HG12084-ANG|
|Human CAT ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12084-ANR|
|Human CAT ORF mammalian expression plasmid, C-Flag tag||HG12084-CF|
|Human CAT ORF mammalian expression plasmid, C-His tag||HG12084-CH|
|Human CAT ORF mammalian expression plasmid, C-Myc tag||HG12084-CM|
|Human CAT ORF mammalian expression plasmid, C-HA tag||HG12084-CY|
|Human CAT Gene cDNA clone plasmid||HG12084-G|
|Human CAT natural ORF mammalian expression plasmid||HG12084-G-N|
|Human CAT ORF mammalian expression plasmid, N-Flag tag||HG12084-NF|
|Human CAT ORF mammalian expression plasmid, N-His tag||HG12084-NH|
|Human CAT ORF mammalian expression plasmid, N-Myc tag||HG12084-NM|
|Human CAT ORF mammalian expression plasmid, N-HA tag||HG12084-NY|
|Human CAT natural ORF mammalian expression plasmid||HG12084-UT|
|Learn more about expression Vectors|
Catalase is a ubiquitously expressed enzyme that catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a tetramer of four polypeptides chains containing four porphyrin heme groups that allow the enzyme to react with the hydrogen peroxide. The optimum PH of human catalase is approximately 7 and the optimum temperature is at 37 degree. Both the PH optimum and temperature for other catalases varies depending on the species. Catalase can be inhibited by a flux of O2- generated in situ by the aerobic xanthine oxidase reaction. This inhibition of catalase by O2- provides the basis for a synergism between superoxide dismutase and catalase.Such synergisms have been observed in vitro and may be significant in vivo. Catalase is used in the food industry for removing hydrogen peroxide from milk prior to cheese production. Another use is in food wrappers where it prevents food from oxidizing. Catalase is also used in the textile industry, removing hydrogen peroxide from fabrics to make sure the material is peroxide-free.