|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PTX3 ORF mammalian expression plasmid, C-GFPSpark tag||HG12082-ACG|
|Human PTX3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12082-ACR|
|Human PTX3 ORF mammalian expression plasmid, C-Flag tag||HG12082-CF|
|Human PTX3 ORF mammalian expression plasmid, C-His tag||HG12082-CH|
|Human PTX3 ORF mammalian expression plasmid, C-Myc tag||HG12082-CM|
|Human PTX3 ORF mammalian expression plasmid, C-HA tag||HG12082-CY|
|Human PTX3 Gene cDNA clone plasmid||HG12082-G|
|Human PTX3 ORF mammalian expression plasmid, N-Flag tag||HG12082-NF|
|Human PTX3 ORF mammalian expression plasmid, N-His tag||HG12082-NH|
|Human PTX3 ORF mammalian expression plasmid, N-Myc tag||HG12082-NM|
|Human PTX3 ORF mammalian expression plasmid, N-HA tag||HG12082-NY|
|Human PTX3 natural ORF mammalian expression plasmid||HG12082-UT|
|Learn more about expression Vectors|
Pentraxin-related protein PTX3, also known as Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, TSG-14, PTX3 and TNFAIP5, is a secreted protein which contains one pentaxin domain. PTX3 plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. Pentraxins are a family of evolutionarily conserved multifunctional pattern-recognition proteins characterized by a cyclic multimeric structure. Based on the primary structure of the subunit, the pentraxins are divided into two groups: short pentraxins and long pentraxins. C-reactive protein (CRP) and serum amyloid P-component (SAP) are the two short pentraxins. The prototype protein of the long pentraxin group is pentraxin 3 (PTX3). CRP and SAP are produced primarily in the liver in response to IL-6, while PTX3 is produced by a variety of tissues and cells and in particular by innate immunity cells in response to proinflammatory signals and Toll-like receptor (TLR) engagement. PTX3 is essential in female fertility by acting as a nodal point for the assembly of the cumulus oophorus hyaluronan-rich extracellular matrix. PTX3 interacts with several ligands, including growth factors, extracellular matrix components and selected pathogens, playing a role in complement activation and facilitating pathogen recognition by phagocytes, acting as a predecessor of antibodies. PTX3 may also contribute to the pathogenesis of atherosclerosis.