|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SCN2B ORF mammalian expression plasmid, C-GFPSpark tag||HG13859-ACG|
|Human SCN2B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13859-ACR|
|Human SCN2B ORF mammalian expression plasmid, C-Flag tag||HG13859-CF|
|Human SCN2B ORF mammalian expression plasmid, C-His tag||HG13859-CH|
|Human SCN2B ORF mammalian expression plasmid, C-Myc tag||HG13859-CM|
|Human SCN2B ORF mammalian expression plasmid, C-HA tag||HG13859-CY|
|Human SCN2B Gene cDNA clone plasmid||HG13859-G|
|Human SCN2B ORF mammalian expression plasmid, N-Flag tag||HG13859-NF|
|Human SCN2B ORF mammalian expression plasmid, N-His tag||HG13859-NH|
|Human SCN2B ORF mammalian expression plasmid, N-Myc tag||HG13859-NM|
|Human SCN2B ORF mammalian expression plasmid, N-HA tag||HG13859-NY|
|Human SCN2B natural ORF mammalian expression plasmid||HG13859-UT|
|Learn more about expression Vectors|
SCN2B plays a key role in the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel. Voltage-gated sodium channels (NaV) are composed of one pore-forming alpha-subunit, which may be associated with either one or more beta-subunits. Alpha-subunits are composed for four homologous domains, each of which contains six transmembrane segments.They are responsible for action potential initiation and propagation in excitable cells, including nerve, muscle, and neuroendocrine cell types. SCN2B causes an increase in the plasma membrane surface area and in its folding into microvilli. SCN2B also interacts with TNR and may play a crucial role in clustering and regulation of activity of sodium channels at nodes of ranvier.