|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human HAO1 ORF mammalian expression plasmid, C-GFPSpark tag||HG12076-ACG|
|Human HAO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12076-ACR|
|Human HAO1 ORF mammalian expression plasmid, N-GFPSpark tag||HG12076-ANG|
|Human HAO1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12076-ANR|
|Human HAO1 ORF mammalian expression plasmid, C-Flag tag||HG12076-CF|
|Human HAO1 ORF mammalian expression plasmid, C-His tag||HG12076-CH|
|Human HAO1 ORF mammalian expression plasmid, C-Myc tag||HG12076-CM|
|Human HAO1 ORF mammalian expression plasmid, C-HA tag||HG12076-CY|
|Human HAO1 Gene cDNA clone plasmid||HG12076-G|
|Human HAO1 ORF mammalian expression plasmid, N-Flag tag||HG12076-NF|
|Human HAO1 ORF mammalian expression plasmid, N-His tag||HG12076-NH|
|Human HAO1 ORF mammalian expression plasmid, N-Myc tag||HG12076-NM|
|Human HAO1 ORF mammalian expression plasmid, N-HA tag||HG12076-NY|
|Human HAO1 natural ORF mammalian expression plasmid||HG12076-UT|
|Learn more about expression Vectors|
Hydroxyacid oxidase 1, also known as Glycolate oxidase, HAO1 and GOX1, is a member of the FMN-dependent alpha-hydroxy acid dehydrogenase family. HAO1 / GOX1 has 2-hydroxyacid oxidase activity. It is most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. HAO1 / GOX1 is a liver-specific peroxisomal enzyme that oxidizes glycolate to glyoxylate with concomitant production of H2O2. In Hao1 messenger RNA (mRNA), an iron-responsive element (IRE) homologous to the sequence recognized by iron regulatory proteins (IRP), key regulators of iron homeostasis, is present. Mammalian HAO1 / GOX1 is a peroxisomal protein and that the C-terminal sequence SKI acts as the targeting signal. Down-regulation of HAO1 / GOX1 expression during oxidative stress may provide a mechanism to prevent excessive H2O2 formation in liver peroxisomes and may represent the prototype of a poorly recognized but potentially relevant response to oxidative injury involving down-regulation of ROS-producing enzymes.