|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human NRAS ORF mammalian expression plasmid, C-GFPSpark tag||HG12073-ACG|
|Human NRAS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12073-ACR|
|Human NRAS ORF mammalian expression plasmid, C-Flag tag||HG12073-CF|
|Human NRAS ORF mammalian expression plasmid, C-His tag||HG12073-CH|
|Human NRAS ORF mammalian expression plasmid, C-Myc tag||HG12073-CM|
|Human NRAS ORF mammalian expression plasmid, C-HA tag||HG12073-CY|
|Human NRAS Gene cDNA clone plasmid||HG12073-G|
|Human NRAS ORF mammalian expression plasmid, N-Flag tag||HG12073-NF|
|Human NRAS ORF mammalian expression plasmid, N-His tag||HG12073-NH|
|Human NRAS ORF mammalian expression plasmid, N-Myc tag||HG12073-NM|
|Human NRAS ORF mammalian expression plasmid, N-HA tag||HG12073-NY|
|Human NRAS natural ORF mammalian expression plasmid||HG12073-UT|
|Learn more about expression Vectors|
NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI).