|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human COL5A2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13685-ACG|
|Human COL5A2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13685-ACR|
|Human COL5A2 ORF mammalian expression plasmid, C-Flag tag||HG13685-CF|
|Human COL5A2 ORF mammalian expression plasmid, C-His tag||HG13685-CH|
|Human COL5A2 ORF mammalian expression plasmid, C-Myc tag||HG13685-CM|
|Human COL5A2 ORF mammalian expression plasmid, C-HA tag||HG13685-CY|
|Human COL5A2 Gene cDNA clone plasmid||HG13685-G|
|Human COL5A2 ORF mammalian expression plasmid, N-Flag tag||HG13685-NF|
|Human COL5A2 ORF mammalian expression plasmid, N-His tag||HG13685-NH|
|Human COL5A2 ORF mammalian expression plasmid, N-Myc tag||HG13685-NM|
|Human COL5A2 ORF mammalian expression plasmid, N-HA tag||HG13685-NY|
|Human COL5A2 natural ORF mammalian expression plasmid||HG13685-UT|
|Learn more about expression Vectors|
COL5A2 is a component of type V collagen. It is known as the pro-alpha2(V) chain. COL5A2, together with two pro-alpha1(V) chains can form type V procollagen. These triple-stranded, rope-like procollagen molecules arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong, mature type V collagen fibers. Type V collagen can be detected in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen.