|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human VWC2 ORF mammalian expression plasmid, C-GFPSpark tag||HG12071-ACG|
|Human VWC2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12071-ACR|
|Human VWC2 ORF mammalian expression plasmid, C-Flag tag||HG12071-CF|
|Human VWC2 ORF mammalian expression plasmid, C-His tag||HG12071-CH|
|Human VWC2 ORF mammalian expression plasmid, C-Myc tag||HG12071-CM|
|Human VWC2 ORF mammalian expression plasmid, C-HA tag||HG12071-CY|
|Human VWC2 Gene cDNA clone plasmid||HG12071-G|
|Human VWC2 ORF mammalian expression plasmid, N-Flag tag||HG12071-NF|
|Human VWC2 ORF mammalian expression plasmid, N-His tag||HG12071-NH|
|Human VWC2 ORF mammalian expression plasmid, N-Myc tag||HG12071-NM|
|Human VWC2 ORF mammalian expression plasmid, N-HA tag||HG12071-NY|
|Human VWC2 natural ORF mammalian expression plasmid||HG12071-UT|
|Learn more about expression Vectors|
Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein which contains two VWFC domains. VWC2 / Brorin is a BMP antagonist which may play a role in neural development. It promotes cell adhesion. VWC2 / Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2 / Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2 / Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.