|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human EPDR1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13665-ACG|
|Human EPDR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13665-ACR|
|Human EPDR1 ORF mammalian expression plasmid, C-Flag tag||HG13665-CF|
|Human EPDR1 ORF mammalian expression plasmid, C-His tag||HG13665-CH|
|Human EPDR1 ORF mammalian expression plasmid, C-Myc tag||HG13665-CM|
|Human EPDR1 ORF mammalian expression plasmid, C-HA tag||HG13665-CY|
|Human EPDR1 Gene cDNA clone plasmid||HG13665-G|
|Human EPDR1 ORF mammalian expression plasmid, N-Flag tag||HG13665-NF|
|Human EPDR1 ORF mammalian expression plasmid, N-His tag||HG13665-NH|
|Human EPDR1 ORF mammalian expression plasmid, N-Myc tag||HG13665-NM|
|Human EPDR1 ORF mammalian expression plasmid, N-HA tag||HG13665-NY|
|Human EPDR1 natural ORF mammalian expression plasmid||HG13665-UT|
|Learn more about expression Vectors|
EPDR1 is a member of the ependymin family. EPDR1 is a type II transmembrane protein that is similar to two families of cell adhesion molecules, the protocadherins and ependymins. It may play a role in calcium-dependent cell adhesion. EPDR1 is glycosylated, and the orthologous mouse protein is localized to the lysosome. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.