|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human STC2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13653-ACG|
|Human STC2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13653-ACR|
|Human STC2 ORF mammalian expression plasmid, C-Flag tag||HG13653-CF|
|Human STC2 ORF mammalian expression plasmid, C-His tag||HG13653-CH|
|Human STC2 ORF mammalian expression plasmid, C-Myc tag||HG13653-CM|
|Human STC2 ORF mammalian expression plasmid, C-HA tag||HG13653-CY|
|Human STC2 Gene cDNA clone plasmid||HG13653-G|
|Human STC2 ORF mammalian expression plasmid, N-Flag tag||HG13653-NF|
|Human STC2 ORF mammalian expression plasmid, N-His tag||HG13653-NH|
|Human STC2 ORF mammalian expression plasmid, N-Myc tag||HG13653-NM|
|Human STC2 ORF mammalian expression plasmid, N-HA tag||HG13653-NY|
|Human STC2 natural ORF mammalian expression plasmid||HG13653-UT|
|Learn more about expression Vectors|
STC2 is a secreted, homodimeric glycoprotein which expressed in a wide variety of tissues. STC2 has an anti-hypocalcemic action on calcium and phosphate homeostasis. It may have autocrine or paracrine functions. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. STC2 has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. It may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis.