|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human LMAN2L ORF mammalian expression plasmid, C-GFPSpark tag||HG13822-ACG|
|Human LMAN2L ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13822-ACR|
|Human LMAN2L ORF mammalian expression plasmid, C-Flag tag||HG13822-CF|
|Human LMAN2L ORF mammalian expression plasmid, C-His tag||HG13822-CH|
|Human LMAN2L ORF mammalian expression plasmid, C-Myc tag||HG13822-CM|
|Human LMAN2L ORF mammalian expression plasmid, C-HA tag||HG13822-CY|
|Human LMAN2L Gene cDNA clone plasmid||HG13822-G|
|Human LMAN2L ORF mammalian expression plasmid, N-Flag tag||HG13822-NF|
|Human LMAN2L ORF mammalian expression plasmid, N-His tag||HG13822-NH|
|Human LMAN2L ORF mammalian expression plasmid, N-Myc tag||HG13822-NM|
|Human LMAN2L ORF mammalian expression plasmid, N-HA tag||HG13822-NY|
|Human LMAN2L natural ORF mammalian expression plasmid||HG13822-UT|
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LMAN2L contains 1 L-type lectin-like domain and is expressed in numerous tissues. It is highly expressed in skeletal muscle and kidney, and its intermediate expression levels in heart, liver and placenta, low levels in brain, thymus, spleen, small intestine and lung. LMAN2L may be involved in the regulation of export from the endoplasmic reticulum of a subset of glycoproteins. It also may function as a regulator of ERGIC-53. LMAN2L gene may be modulated by acetylation; phosphorylation, as detailed at PhosphoSite. Alternative splicing produces 2 isoforms of the human protein. LMAN2L localize in various compartments.