|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human NCKIPSD ORF mammalian expression plasmid, C-GFPSpark tag||HG13642-ACG|
|Human NCKIPSD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13642-ACR|
|Human NCKIPSD ORF mammalian expression plasmid, N-GFPSpark tag||HG13642-ANG|
|Human NCKIPSD ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13642-ANR|
|Human NCKIPSD ORF mammalian expression plasmid, C-Flag tag||HG13642-CF|
|Human NCKIPSD ORF mammalian expression plasmid, C-His tag||HG13642-CH|
|Human NCKIPSD ORF mammalian expression plasmid, C-Myc tag||HG13642-CM|
|Human NCKIPSD ORF mammalian expression plasmid, C-HA tag||HG13642-CY|
|Human NCKIPSD Gene cDNA clone plasmid||HG13642-G|
|Human NCKIPSD ORF mammalian expression plasmid, N-Flag tag||HG13642-NF|
|Human NCKIPSD ORF mammalian expression plasmid, N-His tag||HG13642-NH|
|Human NCKIPSD ORF mammalian expression plasmid, N-Myc tag||HG13642-NM|
|Human NCKIPSD ORF mammalian expression plasmid, N-HA tag||HG13642-NY|
|Human NCKIPSD natural ORF mammalian expression plasmid||HG13642-UT|
|Learn more about expression Vectors|
NCKIPSD is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. NCKIPSD also plays an important role in stress fiber formation. NCKIPSD gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing occurs in this locus and two transcript variants encoding distinct isoforms have been identified. NCKIPSD is a SH3 domain protein. Fas ligand is a cytotoxic effector molecule of T and NK cells which is characterized by an intracellular N-terminal polyproline region that serves as a docking site for SH3 and WW domain proteins. Several previously described Fas ligand-interacting SH3 domain proteins turned out to be crucial for the regulation of storage, expression and function of the death factor. Recent observations, however, indicate that Fas ligand is also subject to posttranslational modifications including shedding and intramembrane proteolysis.