|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human BMF ORF mammalian expression plasmid, C-GFPSpark tag||HG13639-ACG|
|Human BMF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13639-ACR|
|Human BMF ORF mammalian expression plasmid, N-GFPSpark tag||HG13639-ANG|
|Human BMF ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13639-ANR|
|Human BMF ORF mammalian expression plasmid, C-Flag tag||HG13639-CF|
|Human BMF ORF mammalian expression plasmid, C-His tag||HG13639-CH|
|Human BMF ORF mammalian expression plasmid, C-Myc tag||HG13639-CM|
|Human BMF ORF mammalian expression plasmid, C-HA tag||HG13639-CY|
|Human BMF Gene cDNA clone plasmid||HG13639-G|
|Human BMF ORF mammalian expression plasmid, N-Flag tag||HG13639-NF|
|Human BMF ORF mammalian expression plasmid, N-His tag||HG13639-NH|
|Human BMF ORF mammalian expression plasmid, N-Myc tag||HG13639-NM|
|Human BMF ORF mammalian expression plasmid, N-HA tag||HG13639-NY|
|Human BMF natural ORF mammalian expression plasmid||HG13639-UT|
|Learn more about expression Vectors|
BMF(Bcl2 modifying factor) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BMF contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. BMF is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified.