|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SMOC1 ORF mammalian expression plasmid, C-GFPSpark tag||HG12026-ACG|
|Human SMOC1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12026-ACR|
|Human SMOC1 ORF mammalian expression plasmid, C-Flag tag||HG12026-CF|
|Human SMOC1 ORF mammalian expression plasmid, C-His tag||HG12026-CH|
|Human SMOC1 ORF mammalian expression plasmid, C-Myc tag||HG12026-CM|
|Human SMOC1 ORF mammalian expression plasmid, C-HA tag||HG12026-CY|
|Human SMOC1 Gene cDNA clone plasmid||HG12026-G|
|Human SMOC1 ORF mammalian expression plasmid, N-Flag tag||HG12026-NF|
|Human SMOC1 ORF mammalian expression plasmid, N-His tag||HG12026-NH|
|Human SMOC1 ORF mammalian expression plasmid, N-Myc tag||HG12026-NM|
|Human SMOC1 ORF mammalian expression plasmid, N-HA tag||HG12026-NY|
|Human SMOC1 natural ORF mammalian expression plasmid||HG12026-UT|
|Learn more about expression Vectors|
SPARC-related modular calcium-binding protein 1, also known as secreted modular calcium-binding protein 1 and SMOC1, is a member of the SPARC family. SMOC1 is widely expressed in many tissues with a strongest signal in ovary. It contains two EF-hand domains, one Kazal-like domain and two thyroglobulin type-1 domains. Extracellular matrix proteins have been implicated in the regulation of osteoblast differentiation of bone marrow derived mesenchymal stem cells (BMSCs) through paracrine or autocrine mechanisms. SMOC1 is a regulator of osteoblast differentiation of BMSCs. SMOC1 is highly expressed and secreted in BMSCs stimulated with osteogenic medium (OSM). SMOC1 and SMOC2 are matricellular proteins thought to influence growth factor signaling, migration, proliferation, and angiogenesis. SMOC1 and SMOC2 may mediate intercellular signaling and cell type-specific differentiation during gonad and reproductive tract development.