|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human DCBLD2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13615-ACG|
|Human DCBLD2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13615-ACR|
|Human DCBLD2 ORF mammalian expression plasmid, C-Flag tag||HG13615-CF|
|Human DCBLD2 ORF mammalian expression plasmid, C-His tag||HG13615-CH|
|Human DCBLD2 ORF mammalian expression plasmid, C-Myc tag||HG13615-CM|
|Human DCBLD2 ORF mammalian expression plasmid, C-HA tag||HG13615-CY|
|Human DCBLD2 Gene cDNA clone plasmid||HG13615-G|
|Human DCBLD2 ORF mammalian expression plasmid, N-Flag tag||HG13615-NF|
|Human DCBLD2 ORF mammalian expression plasmid, N-His tag||HG13615-NH|
|Human DCBLD2 ORF mammalian expression plasmid, N-Myc tag||HG13615-NM|
|Human DCBLD2 ORF mammalian expression plasmid, N-HA tag||HG13615-NY|
|Human DCBLD2 natural ORF mammalian expression plasmid||HG13615-UT|
|Learn more about expression Vectors|
DCBLD2, also known as ESDN and CLCP1, localizes in various compartments. DCBLD2 is up-regulated in lung cancers and is regulated by transcription factor AP-2 alpha (TFAP2A), a component of activator protein-2 (AP-2) that is known to regulate IL-8 production in human lung fibroblasts and epithelial cells. DCBLD2 could be related to FEV(1)-related phenotypes in asthmatics. DCBLD2 gene is expressed at very high level. DCBLD2 is proposed to participate in processes such as intracellular receptor mediated signaling pathway, negative regulation of cell growth and so on.