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Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-tagged

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TRIB3cDNA Clone Product Information
Gene_bank_ref_id:NM_021158.3
cDNA Size:1077
cDNA Description:ORF Clone of Homo sapiens tribbles homolog 3 (Drosophila) DNA.
Gene Synonym:NIPK, SINK, TRB3, SKIP3, C20orf97, TRIB3
Species:Human
Vector:pCMV3-C-His
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-C-His Vector Information
 
Vector Name pCMV3-C-His
Vector Size 6164bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag His
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-C-His Physical Map

Schematic of pCMV3-C-His Multiple Cloning Sites

His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-tagged on other vectors
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10731-ACG$325
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10731-ACR$325
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10731-ANG$325
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10731-ANR$325
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10731-CF$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10731-CH$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10731-CM$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10731-CY$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone)HG10731-M$95
Human TRIB3 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10731-M-F$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10731-M-N$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10731-NF$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10731-NH$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10731-NM$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10731-NY$295
Human TRIB3 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10731-UT$295
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Background

Tribbles homolog 3, also known as Neuronal cell death-inducible putative kinase, p65-interacting inhibitor of NF-kappa-B, SINK and TRIB3, is a Nucleus protein which belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family and Tribbles subfamily. Highest expression Of TRIB3 is in liver, pancreas, peripheral blood leukocytes and bone marrow. It is also highly expressed in a number of primary lung, colon and breast tumors. TRIB3 is expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. TRIB3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRIB3 may bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. It binds to ATF4 and inhibits its transcriptional activation activity. TRIB3 interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. It interacts with MAPK kinases and regulates activation of MAP kinases. It may play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. TRIB3 does not display kinase activity.

References
  • Wu M. et al., 2003, J. Biol. Chem. 278: 27072-9.
  • Kiss-Toth E. et al., 2004, J. Biol. Chem. 279: 42703-8.
  • Ord D., et al.,2005, Biochem. Biophys. Res. Commun. 330: 210-8.
  • Schwarzer R. et al., 2006, Cell. Signal. 18:899-909.
  • Greenman C. et al., 2007, Nature. 446:153-8.
  • Size / Price
    Catalog:HG10731-CH
    List Price: $295.00  (Save $0.00)
    Price:$295.00      [How to order]
    Availability2-3 weeks
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