|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FGFRL1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13589-ACG|
|Human FGFRL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13589-ACR|
|Human FGFRL1 ORF mammalian expression plasmid, C-Flag tag||HG13589-CF|
|Human FGFRL1 ORF mammalian expression plasmid, C-His tag||HG13589-CH|
|Human FGFRL1 ORF mammalian expression plasmid, C-Myc tag||HG13589-CM|
|Human FGFRL1 ORF mammalian expression plasmid, C-HA tag||HG13589-CY|
|Human FGFRL1 Gene cDNA clone plasmid||HG13589-G|
|Human FGFRL1 ORF mammalian expression plasmid, N-Flag tag||HG13589-NF|
|Human FGFRL1 ORF mammalian expression plasmid, N-His tag||HG13589-NH|
|Human FGFRL1 ORF mammalian expression plasmid, N-Myc tag||HG13589-NM|
|Human FGFRL1 ORF mammalian expression plasmid, N-HA tag||HG13589-NY|
|Human FGFRL1 natural ORF mammalian expression plasmid||HG13589-UT|
|Learn more about expression Vectors|
Fibroblast growth factor receptor-like 1 (FGFRL1) also known as Fibroblast growth factor receptor 5 (FGFR5), is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A unique feature of FGFRL1/FGFR5 is that it does not contain an intracellular tyrosine kinase domain. Some muscle types, including the muscles of the tongue and the diaphragm, express FGFRL1/FGFR5 at relatively high level. In contrast, the heart and the skeletal muscles of the limbs, as well as many other organs (brain, lung, liver, kidney, gut) express Fgfrl1 only at basal level. It is conceivable that FGFRL1/FGFR5 interacts with other Fgfrs, which are expressed in cartilage and muscle, to modulate FGF signaling.