|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Canine IL18 ORF mammalian expression plasmid, C-GFPSpark tag||DG70020-ACG|
|Canine IL18 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70020-ACR|
|Canine IL18 ORF mammalian expression plasmid, C-Flag tag||DG70020-CF|
|Canine IL18 ORF mammalian expression plasmid, C-His tag||DG70020-CH|
|Canine IL18 ORF mammalian expression plasmid, C-Myc tag||DG70020-CM|
|Canine IL18 ORF mammalian expression plasmid, C-HA tag||DG70020-CY|
|Canine IL18 Gene cDNA clone plasmid||DG70020-G|
|Canine IL18 ORF mammalian expression plasmid, N-Flag tag||DG70020-NF|
|Canine IL18 ORF mammalian expression plasmid, N-His tag||DG70020-NH|
|Canine IL18 ORF mammalian expression plasmid, N-Myc tag||DG70020-NM|
|Canine IL18 ORF mammalian expression plasmid, N-HA tag||DG70020-NY|
|Canine IL18 natural ORF mammalian expression plasmid||DG70020-UT|
|Learn more about expression Vectors|
Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.