|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TMEM25 ORF mammalian expression plasmid, C-GFPSpark tag||HG13750-ACG|
|Human TMEM25 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13750-ACR|
|Human TMEM25 ORF mammalian expression plasmid, C-Flag tag||HG13750-CF|
|Human TMEM25 ORF mammalian expression plasmid, C-His tag||HG13750-CH|
|Human TMEM25 ORF mammalian expression plasmid, C-Myc tag||HG13750-CM|
|Human TMEM25 ORF mammalian expression plasmid, C-HA tag||HG13750-CY|
|Human TMEM25 Gene cDNA clone plasmid||HG13750-G|
|Human TMEM25 ORF mammalian expression plasmid, N-Flag tag||HG13750-NF|
|Human TMEM25 ORF mammalian expression plasmid, N-His tag||HG13750-NH|
|Human TMEM25 ORF mammalian expression plasmid, N-Myc tag||HG13750-NM|
|Human TMEM25 ORF mammalian expression plasmid, N-HA tag||HG13750-NY|
|Human TMEM25 natural ORF mammalian expression plasmid||HG13750-UT|
|Learn more about expression Vectors|
TMEM25 is a novel member of the immunoglobulin superfamily. Immunoglobulin superfamily members are implicated in immune responses, growth factor signaling, and cell adhesion. TMEM25 contains 1 Ig-like (immunoglobulin-like) domain and is a target of pharmacogenomics in the field of oncology and regenerative medicine. TMEM25 isoform 1, consisting of exons 1-9, encoded a 366-aa transmembrane protein. TMEM25 isoform 2, consisting of exons 1-4 and 6-9, encoded a 322-aa secreted protein. TMEM25 mRNA was expressed in brain, including cerebellar cortex and hippocampus, as well as in neuroblastoma, brain tumors, and gastric cancer. Human TMEM25 gene was located at the 11q23.3 oncogenomic recombination hotspot around the MLL amplicon and the neuroblastoma deleted region.