|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SMPDL3A ORF mammalian expression plasmid, C-GFPSpark tag||HG13559-ACG|
|Human SMPDL3A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13559-ACR|
|Human SMPDL3A ORF mammalian expression plasmid, C-Flag tag||HG13559-CF|
|Human SMPDL3A ORF mammalian expression plasmid, C-His tag||HG13559-CH|
|Human SMPDL3A ORF mammalian expression plasmid, C-Myc tag||HG13559-CM|
|Human SMPDL3A ORF mammalian expression plasmid, C-HA tag||HG13559-CY|
|Human SMPDL3A Gene cDNA clone plasmid||HG13559-G|
|Human SMPDL3A ORF mammalian expression plasmid, N-Flag tag||HG13559-NF|
|Human SMPDL3A ORF mammalian expression plasmid, N-His tag||HG13559-NH|
|Human SMPDL3A ORF mammalian expression plasmid, N-Myc tag||HG13559-NM|
|Human SMPDL3A ORF mammalian expression plasmid, N-HA tag||HG13559-NY|
|Human SMPDL3A natural ORF mammalian expression plasmid||HG13559-UT|
|Learn more about expression Vectors|
SMPDL3A gene is a novel liver X receptor (LXR) -regulated gene, with an LXR response element within its promoter. The induction of SMPDL3A is LXR-dependent and is restricted to human blood cells with no induction observed in mouse cellular systems. LXR α and LXRβ function as physiological sensors of cholesterol metabolites (oxysterols), regulating key genes involved in cholesterol and lipid metabolism. LXRs have been extensively studied in both human and rodent cell systems, revealing their potential therapeutic value in the contexts of atherosclerosis and inflammatory diseases. The LXR genome landscape has been investigated in murine macrophages but not in human THP-1 cells, which represent one of the frequently used monocyte/macrophage cell systems to study immune responses.