|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human AMIGO2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13735-ACG|
|Human AMIGO2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13735-ACR|
|Human AMIGO2 ORF mammalian expression plasmid, C-Flag tag||HG13735-CF|
|Human AMIGO2 ORF mammalian expression plasmid, C-His tag||HG13735-CH|
|Human AMIGO2 ORF mammalian expression plasmid, C-Myc tag||HG13735-CM|
|Human AMIGO2 ORF mammalian expression plasmid, C-HA tag||HG13735-CY|
|Human AMIGO2 Gene cDNA clone plasmid||HG13735-G|
|Human AMIGO2 ORF mammalian expression plasmid, N-Flag tag||HG13735-NF|
|Human AMIGO2 ORF mammalian expression plasmid, N-His tag||HG13735-NH|
|Human AMIGO2 ORF mammalian expression plasmid, N-Myc tag||HG13735-NM|
|Human AMIGO2 ORF mammalian expression plasmid, N-HA tag||HG13735-NY|
|Human AMIGO2 natural ORF mammalian expression plasmid||HG13735-UT|
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AMIGO2 contains Ig-like C2-type (immunoglobulin-like) domain, 6 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. It belongs to the immunoglobulin superfamily, AMIGO family. AMIGO2 may mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. It is required for depolarization-dependent survival of cultured cerebellar granule neurons. AMIGO2 may also contribute to signal transduction through its intracellular domain. It may play a role in the tumorigenesis of a subset of gastric adenocarcinomas. AMIGO2 is highly expressed in breast, ovary, cervix, and uterus.