|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human LUM ORF mammalian expression plasmid, C-GFPSpark tag||HG11640-ACG|
|Human LUM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11640-ACR|
|Human LUM ORF mammalian expression plasmid, C-Flag tag||HG11640-CF|
|Human LUM ORF mammalian expression plasmid, C-His tag||HG11640-CH|
|Human LUM ORF mammalian expression plasmid, C-Myc tag||HG11640-CM|
|Human LUM ORF mammalian expression plasmid, C-HA tag||HG11640-CY|
|Human LUM Gene cDNA clone plasmid||HG11640-M|
|Human LUM ORF mammalian expression plasmid, N-Flag tag||HG11640-NF|
|Human LUM ORF mammalian expression plasmid, N-His tag||HG11640-NH|
|Human LUM ORF mammalian expression plasmid, N-Myc tag||HG11640-NM|
|Human LUM ORF mammalian expression plasmid, N-HA tag||HG11640-NY|
|Human LUM natural ORF mammalian expression plasmid||HG11640-UT|
|Learn more about expression Vectors|
Lumican, a prototypic leucine-rich proteoglycan with keratan sulfate side chains, is a major component of the cornea, dermal, and muscle connective tissues. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and is also present in the connective tissues of other organs and embryonic corneal stroma as a glycoprotein. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. lumican expressed in injured epithelium may modulate cell behavior such as adhesion or migration, thus contributing to corneal epithelial wound healing. Lumican plays a crucial role in the regulation of collagen assembly into fibrils in various connective tissues and serve as a definitive link between a necessity for lumican in the development of a highly organized collagenous matrix and corneal transparency.