|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ADAMTSL1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13550-ACG|
|Human ADAMTSL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13550-ACR|
|Human ADAMTSL1 ORF mammalian expression plasmid, C-Flag tag||HG13550-CF|
|Human ADAMTSL1 ORF mammalian expression plasmid, C-His tag||HG13550-CH|
|Human ADAMTSL1 ORF mammalian expression plasmid, C-Myc tag||HG13550-CM|
|Human ADAMTSL1 ORF mammalian expression plasmid, C-HA tag||HG13550-CY|
|Human ADAMTSL1 Gene cDNA clone plasmid||HG13550-G|
|Human ADAMTSL1 natural ORF mammalian expression plasmid||HG13550-G-N|
|Human ADAMTSL1 ORF mammalian expression plasmid, N-Flag tag||HG13550-NF|
|Human ADAMTSL1 ORF mammalian expression plasmid, N-His tag||HG13550-NH|
|Human ADAMTSL1 ORF mammalian expression plasmid, N-Myc tag||HG13550-NM|
|Human ADAMTSL1 ORF mammalian expression plasmid, N-HA tag||HG13550-NY|
|Human ADAMTSL1 natural ORF mammalian expression plasmid||HG13550-UT|
|Learn more about expression Vectors|
ADAMTSL1 is a secreted molecule resembling members of the ADAMTS protein family of matrix metalloproteinases. Both ADAMTS proteins and ADAM protein family contain a disintegrin and a metalloprotease domain. Metallospondins is collective term for members of ADAMTS protein family. ADAMTS proteins lack the EGF-like domain found normally in members of the ADAM protein family. They also do not possess the canonical disintegrin sequence found in the ADAM protein family. It contains the domains found in members of the ADAMTS protein family with the exception of the pro-metalloprotease and the disintegrin-like domain typical of this family. ADAMTSL1 gene is expressed in adult skeletal muscle. ADAMTSL1 may play an important role in the extracellular matrix as it is deposited in the cell substratum in a punctate fashion and is excluded from focal contacts.