|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LILRA3 ORF mammalian expression plasmid, C-GFPSpark tag||HG13549-ACG|
|Human LILRA3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13549-ACR|
|Human LILRA3 ORF mammalian expression plasmid, C-Flag tag||HG13549-CF|
|Human LILRA3 ORF mammalian expression plasmid, C-His tag||HG13549-CH|
|Human LILRA3 ORF mammalian expression plasmid, C-Myc tag||HG13549-CM|
|Human LILRA3 ORF mammalian expression plasmid, C-HA tag||HG13549-CY|
|Human LILRA3 Gene cDNA clone plasmid||HG13549-G|
|Human LILRA3 ORF mammalian expression plasmid, N-Flag tag||HG13549-NF|
|Human LILRA3 ORF mammalian expression plasmid, N-His tag||HG13549-NH|
|Human LILRA3 ORF mammalian expression plasmid, N-Myc tag||HG13549-NM|
|Human LILRA3 ORF mammalian expression plasmid, N-HA tag||HG13549-NY|
|Human LILRA3 natural ORF mammalian expression plasmid||HG13549-UT|
|Learn more about expression Vectors|
ILT6, also known as LILRA3, belongs to the ILT family. In human, the ILT gene family includes up to 11 members. The extracellular portion of all members includes at least two and usually four immuno-globulin domains. ILT-2 through 5 are all inhibitory members having variable numbers of cytoplasmic ITIM domains. ILT6 lacks a transmembrane domain. The function of ILT6 is currently unknown. however it is highly homologous to other LILR genes, and can bind human leukocyte antigen (HLA) class I. Therefore, if secreted, the ILT6 might impair interactions of membrane-bound LILRs (such as LILRB1, an inhibitory receptor expressed on effector and memory CD8 T cells) with their HLA ligands, thus modulating immune reactions and influencing susceptibility to disease.