|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human COL5A2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13685-ACG|
|Human COL5A2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13685-ACR|
|Human COL5A2 ORF mammalian expression plasmid, C-Flag tag||HG13685-CF|
|Human COL5A2 ORF mammalian expression plasmid, C-His tag||HG13685-CH|
|Human COL5A2 ORF mammalian expression plasmid, C-Myc tag||HG13685-CM|
|Human COL5A2 ORF mammalian expression plasmid, C-HA tag||HG13685-CY|
|Human COL5A2 Gene cDNA clone plasmid||HG13685-G|
|Human COL5A2 ORF mammalian expression plasmid, N-Flag tag||HG13685-NF|
|Human COL5A2 ORF mammalian expression plasmid, N-His tag||HG13685-NH|
|Human COL5A2 ORF mammalian expression plasmid, N-Myc tag||HG13685-NM|
|Human COL5A2 ORF mammalian expression plasmid, N-HA tag||HG13685-NY|
|Human COL5A2 natural ORF mammalian expression plasmid||HG13685-UT|
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COL5A2 is a component of type V collagen. It is known as the pro-alpha2(V) chain. COL5A2, together with two pro-alpha1(V) chains can form type V procollagen. These triple-stranded, rope-like procollagen molecules arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong, mature type V collagen fibers. Type V collagen can be detected in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen.