|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SNAP25 ORF mammalian expression plasmid, C-GFPSpark tag||HG11585-ACG|
|Human SNAP25 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11585-ACR|
|Human SNAP25 ORF mammalian expression plasmid, N-GFPSpark tag||HG11585-ANG|
|Human SNAP25 ORF mammalian expression plasmid, C-Flag tag||HG11585-CF|
|Human SNAP25 ORF mammalian expression plasmid, C-His tag||HG11585-CH|
|Human SNAP25 ORF mammalian expression plasmid, C-Myc tag||HG11585-CM|
|Human SNAP25 ORF mammalian expression plasmid, C-HA tag||HG11585-CY|
|Human SNAP25 Gene cDNA clone plasmid||HG11585-M|
|Human SNAP25 ORF mammalian expression plasmid, N-Flag tag||HG11585-NF|
|Human SNAP25 ORF mammalian expression plasmid, N-His tag||HG11585-NH|
|Human SNAP25 ORF mammalian expression plasmid, N-Myc tag||HG11585-NM|
|Human SNAP25 ORF mammalian expression plasmid, N-HA tag||HG11585-NY|
|Human SNAP25 natural ORF mammalian expression plasmid||HG11585-UT|
|Learn more about expression Vectors|
Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein which belongs to the SNAP-25 family. SNAP25 / SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25 / SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 / SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25 / SUP is a Q-SNARE protein contributing two α-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25 / SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25 / SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25 / SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25 / SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25 / SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.