|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CALU ORF mammalian expression plasmid, C-GFPSpark tag||HG13486-ACG|
|Human CALU ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13486-ACR|
|Human CALU ORF mammalian expression plasmid, C-Flag tag||HG13486-CF|
|Human CALU ORF mammalian expression plasmid, C-His tag||HG13486-CH|
|Human CALU ORF mammalian expression plasmid, C-Myc tag||HG13486-CM|
|Human CALU ORF mammalian expression plasmid, C-HA tag||HG13486-CY|
|Human CALU Gene cDNA clone plasmid||HG13486-G|
|Human CALU ORF mammalian expression plasmid, N-Flag tag||HG13486-NF|
|Human CALU ORF mammalian expression plasmid, N-His tag||HG13486-NH|
|Human CALU ORF mammalian expression plasmid, N-Myc tag||HG13486-NM|
|Human CALU ORF mammalian expression plasmid, N-HA tag||HG13486-NY|
|Human CALU natural ORF mammalian expression plasmid||HG13486-UT|
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Calumenin belongs to the CREC family. It contains 6 EF-hand domains. Calumenin is expressed in skeletal muscle (at protein level). Calumenin interacts with GGCX and RYR1 in the presence of calcium ions, but not in the presence of EDTA. Calumenin is Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. It seems to inhibit gamma-carboxylase GGCX. Calumenin also binds 7 calcium ions with a low affinity and may modulate calcium release from the sarcoplasmic reticulum.