|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CST1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11568-ACG|
|Human CST1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11568-ACR|
|Human CST1 ORF mammalian expression plasmid, C-Flag tag||HG11568-CF|
|Human CST1 ORF mammalian expression plasmid, C-His tag||HG11568-CH|
|Human CST1 ORF mammalian expression plasmid, C-Myc tag||HG11568-CM|
|Human CST1 ORF mammalian expression plasmid, C-HA tag||HG11568-CY|
|Human CST1 Gene cDNA clone plasmid||HG11568-M|
|Human CST1 ORF mammalian expression plasmid, N-Flag tag||HG11568-NF|
|Human CST1 ORF mammalian expression plasmid, N-His tag||HG11568-NH|
|Human CST1 ORF mammalian expression plasmid, N-Myc tag||HG11568-NM|
|Human CST1 ORF mammalian expression plasmid, N-HA tag||HG11568-NY|
|Human CST1 natural ORF mammalian expression plasmid||HG11568-UT|
|Learn more about expression Vectors|
Cystatin-SN, also known as Cystain-SA-I, Cystatin-1, Salivary cystatin-SA-1 and CST1, is a secreted protein which belongs to the cystatin family. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. Cystatin-SN / CST1 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Cystatin-SN / CST1 is also expressed in saliva, tears, urine and seminal fluid. Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Human salivary cystatins include Cystatin-S, Cystatin-S1, Cystatin-S2, Cystatin- SA, Cystatin-SN, Cystatin-C and Cystatin-D. Cystatin-SN is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin-S, although both inhibit ficin equally well.