|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human STC2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13653-ACG|
|Human STC2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13653-ACR|
|Human STC2 ORF mammalian expression plasmid, C-Flag tag||HG13653-CF|
|Human STC2 ORF mammalian expression plasmid, C-His tag||HG13653-CH|
|Human STC2 ORF mammalian expression plasmid, C-Myc tag||HG13653-CM|
|Human STC2 ORF mammalian expression plasmid, C-HA tag||HG13653-CY|
|Human STC2 Gene cDNA clone plasmid||HG13653-G|
|Human STC2 ORF mammalian expression plasmid, N-Flag tag||HG13653-NF|
|Human STC2 ORF mammalian expression plasmid, N-His tag||HG13653-NH|
|Human STC2 ORF mammalian expression plasmid, N-Myc tag||HG13653-NM|
|Human STC2 ORF mammalian expression plasmid, N-HA tag||HG13653-NY|
|Human STC2 natural ORF mammalian expression plasmid||HG13653-UT|
|Learn more about expression Vectors|
STC2 is a secreted, homodimeric glycoprotein which expressed in a wide variety of tissues. STC2 has an anti-hypocalcemic action on calcium and phosphate homeostasis. It may have autocrine or paracrine functions. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. STC2 has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. It may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis.