|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SRGN ORF mammalian expression plasmid, C-GFPSpark tag||HG13648-ACG|
|Human SRGN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13648-ACR|
|Human SRGN ORF mammalian expression plasmid, C-Flag tag||HG13648-CF|
|Human SRGN ORF mammalian expression plasmid, C-His tag||HG13648-CH|
|Human SRGN ORF mammalian expression plasmid, C-Myc tag||HG13648-CM|
|Human SRGN ORF mammalian expression plasmid, C-HA tag||HG13648-CY|
|Human SRGN Gene cDNA clone plasmid||HG13648-G|
|Human SRGN ORF mammalian expression plasmid, N-Flag tag||HG13648-NF|
|Human SRGN ORF mammalian expression plasmid, N-His tag||HG13648-NH|
|Human SRGN ORF mammalian expression plasmid, N-Myc tag||HG13648-NM|
|Human SRGN ORF mammalian expression plasmid, N-HA tag||HG13648-NY|
|Human SRGN natural ORF mammalian expression plasmid||HG13648-UT|
|Learn more about expression Vectors|
SRGN is known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of various hematopoietic cells has a protease-resistant peptide core, and is vital for neutralizing hydrolytic enzymes. SRGN is associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. It is required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. SRGN also plays a role in localizing neutrophil elastase in azurophil granules of neutrophils.