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Danio rerio (zebrafish) BMP-2 / BMP2A Protein

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Danio rerio (zebrafish) BMP-2 Protein Product Information
Synonym:BMP2A
Protein Construction:A DNA sequence encoding the zebrafish BMP2A (B3DI86) (Gln272-Arg386) was expressed and purified with an initial Met.
Species:Danio rerio (zebrafish)
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Danio rerio (zebrafish) BMP-2 Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
The ED50 for this effect is typically 0.5-3 μg/mL.
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant zebrafish BMP2A consists of 116 amino acids and has a calculated molecular mass of 13.2 kDa. It migrates as an 13 KDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 30mM HAC, pH 3.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Danio rerio (zebrafish) BMP-2 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Danio rerio (zebrafish) BMP-2 Protein SDS-PAGE
Danio rerio (zebrafish) BMP-2 / BMP2A Protein SDS-PAGE
Other BMP-2 Recombinant Protein Products
63003-ZNAE
BMP-2  Background

BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.

Danio rerio (zebrafish) BMP-2  References
  • Jiao X, et al. (2007) Heparan sulfate proteoglycans (HSPGs) modulate BMP-2 osteogenic bioactivity in C2C12 cells. J Biol Chem. 282(2):1080-6.
  • Michon F, et al. (2008) BMP-2 and BMP-7 play antagonistic roles in feather induction. Development 135 (16):2797-805.
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    Catalog: 63003-ZNAE-5
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"