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Danio rerio (zebrafish) EFNB2A / Ephrin B2a Protein (His Tag)

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Danio rerio (zebrafish) EFNB2A Protein Product Information
Synonym:EFNB2A, efnb2
Protein Construction:A DNA sequence encoding the zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with a polyhistidine tag at the C-terminus.
Species:Danio rerio (zebrafish)
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Danio rerio (zebrafish) EFNB2A Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:Measured by its binding ability in a functional ELISA.
Immobilized zebrafish EFNB2A-His at 10 μg/ml (100 μl/well) can bind human EphB4-Fc (Cat:10235-H02H), The EC50 of human EphB4-Fc (Cat:10235-H02H) is 15.5-36.3 ng/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Leu 25
Molecule Mass:The recombinant zebrafish EFNB2A consists of 209 amino acids and has a calculated molecular mass of 23.3 kDa. It migrates as an 35-40 KDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, PH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Danio rerio (zebrafish) EFNB2A Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Danio rerio (zebrafish) EFNB2A Protein SDS-PAGE
Danio rerio (zebrafish) EFNB2A / Ephrin B2a Protein (His Tag) SDS-PAGE
Other EFNB2A Recombinant Protein Products
63002-Z08H
EFNB2A / Ephrin B2a Background

Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.

Danio rerio (zebrafish) EFNB2A / Ephrin B2a References
  • Shaw KM. et al., 2006, Dev Dyn. 235 (7): 1753-60.
  • Kemp HA. et al., 2009, Dev Biol. 327 (2): 313-26.
  • Patten SA. et al., 2012, PLoS One. 7 (2): e31650.
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    Catalog: 63002-Z08H-100
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"