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Danio rerio (zebrafish) EFNB2A / Ephrin B2a HEK293 Cell Lysate (WB positive control)

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Danio rerio (zebrafish) EFNB2A Transfected / Overexpression Cell Lysate Product Information
Expressed Host:Human Cells
Product Description:Human Cell lysate that Danio rerio (zebrafish) EFNB2A / Ephrin B2a transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Sequence information:A DNA sequence encoding the zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:Leu 25
Molecule Mass:The recombinant zebrafish EFNB2A consists of 209 amino acids and has a calculated molecular mass of 23.3 kDa. It migrates as an 35-40 KDa band in SDS-PAGE under reducing conditions.
Species:Danio rerio (zebrafish)
Danio rerio (zebrafish) EFNB2A Transfected / Overexpression Cell Lysate Usage Guide
Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Stability:Samples are stable for up to twelve months from date of receipt.
Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
Application notes:Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
EFNB2A / Ephrin B2a Background

Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.

Danio rerio (zebrafish) EFNB2A / Ephrin B2a References
  • Shaw KM. et al., 2006, Dev Dyn. 235 (7): 1753-60.
  • Kemp HA. et al., 2009, Dev Biol. 327 (2): 313-26.
  • Patten SA. et al., 2012, PLoS One. 7 (2): e31650.
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    Catalog: 63002-Z08HL-300
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