|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CLPS ORF mammalian expression plasmid, C-GFPSpark tag||HG13631-ACG|
|Human CLPS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13631-ACR|
|Human CLPS ORF mammalian expression plasmid, C-Flag tag||HG13631-CF|
|Human CLPS ORF mammalian expression plasmid, C-His tag||HG13631-CH|
|Human CLPS ORF mammalian expression plasmid, C-Myc tag||HG13631-CM|
|Human CLPS ORF mammalian expression plasmid, C-HA tag||HG13631-CY|
|Human CLPS Gene cDNA clone plasmid||HG13631-G|
|Human CLPS ORF mammalian expression plasmid, N-Flag tag||HG13631-NF|
|Human CLPS ORF mammalian expression plasmid, N-His tag||HG13631-NH|
|Human CLPS ORF mammalian expression plasmid, N-Myc tag||HG13631-NM|
|Human CLPS ORF mammalian expression plasmid, N-HA tag||HG13631-NY|
|Human CLPS natural ORF mammalian expression plasmid||HG13631-UT|
|Learn more about expression Vectors|
Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.