|Datasheet||Specific References||Reviews||Related Products||Protocols|
|A DNA sequence encoding the canine EGFR (XP_533073.3) (Met1-Ser618) was expressed with a C-terminal polyhistidine tag.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 90 % as determined by SDS-PAGE|
|Measured by its binding ability in a functional ELISA.|
Immobilized canine EGFR-His at 10 μg/ml (100 μl/well) can bind biotinylated canine His-EGF(Cat:70013-D07E), The EC50 of biotinylated canine His-EGF(Cat:70013-D07E) is 0.16-0.36μg/ml
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant canine EGFR comprises 629 amino acids and has a predicted molecular mass of 69.8 kDa. The apparent molecular mass of the protein is approximately 87.2 kDa in SDS-PAGE under reducing conditions.|
|Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0.|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.